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KMID : 0363020050350020289
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Journal of Korean Academy of Periodontology
2005 Volume.35 No. 2 p.289 ~ p.298
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Effect of BMP-7 on the rat periodontal Ligament cell
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김경희
김영준/정현주
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Abstract
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Bone morphogenetic protein-7(BMP-7), a member of the transforming growth factor
superfamily, stimulates osteoblast differentiation and bone formation. There are lots of
evidences supporting a direct participation of periodontal ligament(PDL) cells on periodontal
tissue regeneration. The purpose of this study was to evaluate the effect of recombinant
human(rh) BMP-7 on primary rat PDL cells in vitro, with special focus on the ability of
bone formation. The PDL cells were cultured with rhBMP-7 at the concentration of 0, 10,
25, 50, 100 and 200ng/㎖ for MTT assay. We evaluated the alkaline phosphatase activity at
3 and 5 days of incubation and the ability to produce mineralized nodules of rat PDL cells at
14 days of cell culture in concentration of 0, 10, 25, 50 and 100ng/㎖.
The cell activity was not reduced in cells treated with BMP-7 at 10～100ng/㎖, whereas
the cell activity was reduced in the concentration of 200ng/㎖ than the control at day 1 and
3(p<0.01).
At 3 and 5 day, alkaline phosphatase activity was significantly increased in cells treated
with BMP-7 at 50ng/㎖ and 100ng/㎖(p<0.05). The area of mineralized bone nodule was
greater in cells treated with BMP-7 at 50 and 100 ng/㎖ than the control(p<0.01).
These results suggest that rhBMP-7 stimulate rat PDL cells to differentiate toward
osteoblast phenotype and secretion of the extracellular matrix of rat PDL cells.
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KEYWORD
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rat periodontal Ligament cells, BMP-7, alkaline phosphatase, mineralized nodule
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